Wellcome Trust Centre For Mitochondrial Research

Professor Mary Herbert

Personal Biography

M-Herbert216As Professor of Reproductive Biology with an Honorary Consultant Clinical Scientist post with Newcastle upon Tyne Hospitals NHS Foundation Trust, I lead a team of clinical and research scientists working side by side in a programme of basic, translational and clinical science.

Research Biography

The close integration of clinical and research scientists is a cornerstone of the research programme in my lab. We aim to advance knowledge in the biology of germ cells and early embryos with a view to (i) improving the treatment and understanding of infertility (ii) extending the scope of reproductive technologies to prevent transmission of disease.

Research Interests

The fertilised zygote inherits its nuclear DNA from the male and female gametes – the oocyte and the sperm, which become haploid during a specialised form of cell division known as meiosis. By contrast, mitochondrial DNA is inherited only from the oocyte, which contains some hundreds of thousands of copies. Our research encompasses the inheritance of nuclear and mitochondrial DNA.

Recent Key publications

Herbert M, Kalleas D, Cooney D, Lamb M, Lister LM. Meiosis and maternal ageing: Insights from aneuploid oocytes and trisomy births.  Cold Spring Harb. Perspect. Biol. 2015;7:a017970

Pyle A, Hudson G, Wilson IJ, Coxhead J, Smertenko T, Herbert M, Santibanez-Koref M, Chinnery PF. Extreme-depth re-sequencing finds no evidence of paternal transmission in humans.  PLoS Genet. 2015;11:e1005040

Gorman GS*, Grady JP*, Ng Y, Schaefer AM, McNally RJ, Chinnery PF, Yu Wai Man P, Herbert M, Taylor RW, McFarland RM, Turnbull DM. Mitochondrial Donation: How many women could benefit. N. Engl. J. Med. 2015;372:885-887.

Richardson J, Irving L, Hyslop LA, Choudhary M, Murdoch A, Turnbull DM, Herbert M. Assisted reproductive technologies to prevent transmission of mitochondrial DNA disease. Stem Cells 2015;33:639-645.

Greggains GD, Lister LM, Tuppen HA, Zhang Q, Needham LH, Prathalingam N, Hyslop LA, Craven L, Polanski Z, Murdoch AP, Turnbull DM, Herbert M. Therapeutic potential of somatic cell nuclear transfer for degenerative disease caused by mitochondrial DNA mutations. Sci. Report. 2014;24:3844.

Touati SA, Cladière D, Lister LM, Leontiou I, Chambon JP, Rattani A, Böttger F, Stemmann O, Nasmyth K, Herbert M, Wassmann K. Cyclin A2 Is Required for Sister Chromatid Segregation, But Not Separase Control, in Mouse Oocyte Meiosis. Cell Reports 2012;2:1077-1087.

Prathalingam N. Ferguson L, Young, L, Lietz G, Oldershaw R, Healey L, Craig A, Binaykia R, Seth R, Lister H, Murdoch A, Herbert M. Production and validation of a GMP grade human fibroblast line for supporting human embryonic stem cell derivation and culture. Stem Cell Res. Ther. 2012;3:12.

Choudhary M, Nesbitt M, Burgess L, Hyslop L, Herbert M, Murdoch AP. Egg sharing for research: A successful outcome for patients and researchers. Cell Stem Cell 2012;10:239-240.

Lister LM, Kouznetsova A, Hyslop LA, Kalleas D, Pace SL, Barel JC, Nathan A, Floros V, Adelfalk C, Watanabe Y, Jessberger R, Kirkwood TB, Hoog C, Herbert M.  Age-related meiotic segregation errors in mammalian oocytes are preceded by depletion of cohesin and Sgo2. Current Biol. 2010;20:1511-1521.

Craven L, Tuppen HA, Greggains GD, Harbottle SJ, Murphy JL, Cree LM, Murdoch AP, Chinnery PF, Taylor RW, Lightowlers RN, Herbert M*, Turnbull DM*.  Pronuclear transfer in human embryos to prevent transmission of mitochondrial DNA disease. Nature 2010;465:82-85. (* joint corresponding authors)

 

Publications

Hyslop LA; Blakeley P; Craven L; Richardson J; Fogarty NM; Fragouli E; Lamb M; Wamaitha SE; Prathalingam N; Zhang Q; O'Keefe H; Takeda Y; Arizzi L; Alfarawati S; Tuppen HA; Irving L; Kalleas D; Choudhary M; Wells D; Murdoch AP; Turnbull DM; Niakan KK; Herbert M, 2016. Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease.  Nature.

Craven L; Herbert M; Murdoch A; Murphy J; Lawford Davies J; Turnbull DM, 2016. Research into Policy: A Brief History of Mitochondrial Donation.  Stem Cells 34(2):265-7

Gorman GS; Grady JP; Ng Y; Schaefer AM; McNally RJ; Chinnery PF; Yu-Wai-Man P; Herbert M; Taylor RW; McFarland R; Turnbull DM, 2015. Mitochondrial donation--how many women could benefit?  N Engl J Med 372(9):885-7

Richardson J; Irving L; Hyslop LA; Choudhary M; Murdoch A; Turnbull DM; Herbert M, 2015. Concise reviews: Assisted reproductive technologies to prevent transmission of mitochondrial DNA disease.  Stem Cells 33(3):639-45

Herbert M; Kalleas D; Cooney D; Lamb M; Lister L, 2015. Meiosis and maternal aging: insights from aneuploid oocytes and trisomy births.  Cold Spring Harb Perspect Biol 7(4):a017970

Pyle A; Hudson G; Wilson IJ; Coxhead J; Smertenko T; Herbert M; Santibanez-Koref M; Chinnery PF, 2015. Extreme-Depth Re-sequencing of Mitochondrial DNA Finds No Evidence of Paternal Transmission in Humans.  PLoS Genet 11(5):e1005040

Panagiotopoulou N; Ghuman N; Sandher R; Herbert M; Stewart JA, 2015. Barriers and facilitators towards fertility preservation care for cancer patients: a meta-synthesis.  Eur J Cancer Care (Engl).

Herbert M; Turnbull D, 2015. Mitochondrial replacement to prevent the transmission of mitochondrial DNA disease.  EMBO Rep 16(5):539-40

Greggains GD; Lister LM; Tuppen HA; Zhang Q; Needham LH; Prathalingam N; Hyslop LA; Craven L; Polanski Z; Murdoch AP; Turnbull DM; Herbert M, 2014. Therapeutic potential of somatic cell nuclear transfer for degenerative disease caused by mitochondrial DNA mutations.  Sci Rep 4:3844

Chinnery PF; Craven L; Mitalipov S; Stewart JB; Herbert M; Turnbull DM, 2014. The challenges of mitochondrial replacement.  PLoS Genet 10(4):e1004315

Hyslop L; Prathalingam N; Nowak L; Fenwick J; Harbottle S; Byerley S; Rhodes J; Watson B; Henderson R; Murdoch A; Herbert M, 2012. A novel isolator-based system promotes viability of human embryos during laboratory processing.  PLoS One 7(2):e31010

Prathalingam N; Ferguson L; Young L; Lietz G; Oldershaw R; Healy L; Craig A; Lister H; Binaykia R; Sheth R; Murdoch A; Herbert M, 2012. Production and validation of a good manufacturing practice grade human fibroblast line for supporting human embryonic stem cell derivation and culture.  Stem Cell Res Ther 3(2):12

Craven L; Elson JL; Irving L; Tuppen HA; Lister LM; Greggains GD; Byerley S; Murdoch AP; Herbert M; Turnbull D, 2011. Mitochondrial DNA disease: new options for prevention.  Hum Mol Genet 20(R2):R168-74

Craven L; Tuppen HA; Greggains GD; Harbottle SJ; Murphy JL; Cree LM; Murdoch AP; Chinnery PF; Taylor RW; Lightowlers RN; Herbert M; Turnbull DM, 2010. Pronuclear transfer in human embryos to prevent transmission of mitochondrial DNA disease.  Nature 465(7294):82-5

Lister LM; Kouznetsova A; Hyslop LA; Kalleas D; Pace SL; Barel JC; Nathan A; Floros V; Adelfalk C; Watanabe Y; Jessberger R; Kirkwood TB; Höög C; Herbert M, 2010. Age-related meiotic segregation errors in mammalian oocytes are preceded by depletion of cohesin and Sgo2.  Curr Biol 20(17):1511-21

Nayernia K; Lee JH; Lako M; Armstrong L; Herbert M; Li M; Engel W; Elliott D; Stojkovic M; Parrington J; Murdoch A; Strachan T; Zhang X, 2009. RETRACTION - In Vitro Derivation of Human Sperm from Embryonic Stem Cells.  Stem Cells Dev.

Hall VJ; Compton D; Stojkovic P; Nesbitt M; Herbert M; Murdoch A; Stojkovic M, 2007. Developmental competence of human in vitro aged oocytes as host cells for nuclear transfer.  Hum Reprod 22(1):52-62

Choudhary M; Zhang X; Stojkovic P; Hyslop L; Anyfantis G; Herbert M; Murdoch AP; Stojkovic M; Lako M, 2007. Putative role of hyaluronan and its related genes, HAS2 and RHAMM, in human early preimplantation embryogenesis and embryonic stem cell characterization.  Stem Cells 25(12):3045-57

Jayaprakasan K; Herbert M; Moody E; Stewart JA; Murdoch AP, 2007. Estimating the risks of ovarian hyperstimulation syndrome (OHSS): implications for egg donation for research.  Hum Fertil (Camb) 10(3):183-7

Brown DT; Herbert M; Lamb VK; Chinnery PF; Taylor RW; Lightowlers RN; Craven L; Cree L; Gardner JL; Turnbull DM, 2006. Transmission of mitochondrial DNA disorders: possibilities for the future.  Lancet 368(9529):87-9

Feenan K; Herbert M, 2006. Can 'abnormally' fertilized zygotes give rise to viable embryos?  Hum Fertil (Camb) 9(3):157-69

Homer HA; McDougall A; Levasseur M; Yallop K; Murdoch AP; Herbert M, 2005. Mad2 prevents aneuploidy and premature proteolysis of cyclin B and securin during meiosis I in mouse oocytes.  Genes Dev 19(2):202-7

Homer HA; McDougall A; Levasseur M; Murdoch AP; Herbert M, 2005. Mad2 is required for inhibiting securin and cyclin B degradation following spindle depolymerisation in meiosis I mouse oocytes.  Reproduction 130(6):829-43

Homer HA; McDougall A; Levasseur M; Herbert M, 2005. Restaging the spindle assembly checkpoint in female mammalian meiosis I.  Cell Cycle 4(5):650-3

Homer HA; McDougall A; Levasseur M; Murdoch AP; Herbert M, 2005. RNA interference in meiosis I human oocytes: towards an understanding of human aneuploidy.  Mol Hum Reprod 11(6):397-404

Stojkovic M; Stojkovic P; Leary C; Hall VJ; Armstrong L; Herbert M; Nesbitt M; Lako M; Murdoch A, 2005. Derivation of a human blastocyst after heterologous nuclear transfer to donated oocytes.  Reprod Biomed Online 11(2):226-31

Hyslop L; Stojkovic M; Armstrong L; Walter T; Stojkovic P; Przyborski S; Herbert M; Murdoch A; Strachan T; Lako M, 2005. Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages.  Stem Cells 23(8):1035-43

Stojkovic M; Lako M; Stojkovic P; Stewart R; Przyborski S; Armstrong L; Evans J; Herbert M; Hyslop L; Ahmad S; Murdoch A; Strachan T, 2004. Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture.  Stem Cells 22(5):790-7

Choudhary M; Haimes E; Herbert M; Stojkovic M; Murdoch AP, 2004. Demographic, medical and treatment characteristics associated with couples' decisions to donate fresh spare embryos for research.  Hum Reprod 19(9):2091-6

Madgwick S; Nixon VL; Chang HY; Herbert M; Levasseur M; Jones KT, 2004. Maintenance of sister chromatid attachment in mouse eggs through maturation-promoting factor activity.  Dev Biol 275(1):68-81

Herbert M; Levasseur M; Homer H; Yallop K; Murdoch A; McDougall A, 2003. Homologue disjunction in mouse oocytes requires proteolysis of securin and cyclin B1.  Nat Cell Biol 5(11):1023-5

Fenwick J; Platteau P; Murdoch AP; Herbert M, 2002. Time from insemination to first cleavage predicts developmental competence of human preimplantation embryos in vitro.  Hum Reprod 17(2):407-12

Clouston HJ; Herbert M; Fenwick J; Murdoch AP; Wolstenholme J, 2002. Cytogenetic analysis of human blastocysts.  Prenat Diagn 22(12):1143-52

Griffiths TA; Murdoch AP; Herbert M, 2000. Embryonic development in vitro is compromised by the ICSI procedure.  Hum Reprod 15(7):1592-6

Evbuomwan IO; Fenwick JD; Shiels R; Herbert M; Murdoch AP, 1999. Severe ovarian hyperstimulation syndrome following salvage of empty follicle syndrome.  Hum Reprod 14(7):1707-9

Clouston HJ; Fenwick J; Webb AL; Herbert M; Murdoch A; Wolstenholme J, 1997. Detection of mosaic and non-mosaic chromosome abnormalities in 6- to 8-day old human blastocysts.  Hum Genet 101(1):30-6

Herbert M; Wolstenholme J; Murdoch AP; Butler TJ, 1995. Mitotic activity during preimplantation development of human embryos.  J Reprod Fertil 103(2):209-14

Herbert M; Murdoch AP; Gillespie JI, 1995. The thiol reagent, thimerosal induces intracellular calcium oscillations in mature human oocytes.  Hum Reprod 10(8):2183-6

Publication list retrieved from NCBI using ImpactPubs

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